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    Your Position: Home > Services > Protein Expression Services > E.coli Protein Expression Service
    E.coli Protein Expression Service

    E. coli protein expression system is the most widely used protein expression system, serves as an attractive host system due to its well-characterized genetic background, high yield capacity, robust production cycle, strong anti-pollution abilities, and ease of scaling, suitable for protein expression of many genera. However, the E. Coli protein expression system also faces some limitations, such as hard to express, formation of inclusion bodies and endotoxin. As a leading protein antibody service supplier, Clongene has more than ten years of experience in protein production and can solve various problems in the process of protein expression to meet the needs of customers.

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    Service Advantages
  • One-stop service: from codon optimization to protein expression purification
  • Rich experience: more than 450 kinds of protein purification and protein regenerating experience
  • High success rate: the success rate of expression is higher than 95%
  • Additional services: provide label removal, endotoxin removal, protein labeling and other services
  • E.coli Protein Expression Service Details
    Service StepService DescriptionTimelineDeliverable

    Gene synthesis and subclone

      - Codon optimization and gene synthesis

      - Subcloning into an expression vector

      - Plasmid amplification and preparation

    1-2 weeks

      - Purified proteins 

      - CoA

    Expression identification and solubility analysis

      - 10mL culture

      - Expression conditions groping

      - Protein expression evaluation (SDS-PAGE)

    1-2 weeks
    Protein expression and purification

      - Protein purification (soluble expression)

      - Protein denaturation and renaturation purification (inclusion bodies)

      - QC analysis 

    2-3 weeks
    E.coli Expression Service Process
  • Gene synthesis
  • Vector construction and plasmid amplification
  • Pilot expression and purification study
  • Feedback pilot expression and purification study results
  • Protein expression amplification and purification
  • Product delivery
  • E.coli Expression Case
    Case #1: Expression of the target protein in E.coli

    According to the customer's requirements, the target protein is expressed in E.coli, and purified  by Ni-NTA affinity chromatography, the SDS-PAGE is shown in Figure 1 and Figure 2.

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  • Phone: +86-571-88617879
  • Email: cro_service@clongene.com