Baculovirus-Insect expression system is one of the widely used eukaryotic expression systems at present. Compared with the E.coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. Clongene can provide high-quality experimental scheme and significantly improve the yield of your target protein, including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
| Gene synthesis and subclone | - Codon optimization and gene synthesis - Clone the target gene into the vector plasmid | 1-2 weeks | - Purified proteins - CoA |
| Construction of recombinant baculovirus vector | - Subcloning the target gene into a baculovirus transfer vector | 1-2 weeks | |
Recombinant baculovirus expansion culture and protein expression and purification | - Transfect insect cells, prepare viral stocks and expand cultures - Pilot expression and purification trial - QC analysis | 2-3 weeks | |
Scale up expression and purification (optional) | - Target protein - QC analysis | 3-5 weeks |
Use the baculovirus-insect cell expression system to express and purify the target protein. The SDS-PAGE is as follows:
