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    Your Position: Home > Services > Protein Expression Services > Baculovirus-Insect Cell Protein Expression Service
    Baculovirus-Insect Cell Protein Expression Service

    Baculovirus-Insect expression system is one of the widely used eukaryotic expression systems at present. Compared with the E.coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. Clongene can provide high-quality experimental scheme and significantly improve the yield of your target protein, including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.


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    Baculovirus-Insect Cell Protein Expression Service Details

    Service StepService   DescriptionTimelineDeliverable
    Gene synthesis and subclone  - Codon optimization and gene synthesis

      - Clone the   target gene into the vector plasmid

    1-2 weeks

      - Purified proteins

      - CoA 
    Construction of recombinant baculovirus vector  - Subcloning the target gene into a baculovirus transfer vector1-2 weeks

    Recombinant baculovirus expansion culture and

    protein expression and purification

      - Transfect insect cells, prepare viral stocks and expand cultures

      - Pilot expression and purification trial

      - QC analysis
    2-3 weeks

    Scale up expression and purification (optional)

      - Target protein

      - QC analysis
    3-5 weeks

    Baculovirus Protein Expression Service Workflow
  • Gene synthesis
  • Clone the target gene into the vector plasmid
  • Cloning the target gene into the baculovirus transfer vector by transposition
  • Transfection of insect cells and virus expansion
  • Small scale pilot expression and purification
  • Scale up expression and purification (optional)
  • Product delivery
  • Baculovirus Protein Expression Case
    Case #1: Express the target protein in insect cells

    Use the baculovirus-insect cell expression system to express and purify the target protein. The  SDS-PAGE is as follows:

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  • Phone: +86-571-88617879
  • Email: cro_service@clongene.com