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    Your Position: Home > Services > Protein Expression Services > Baculovirus-Insect Cell Protein Expression Service
    Baculovirus-Insect Cell Protein Expression Service

    Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. Compared to the E. coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. Clongene can provide high-quality experimental scheme and significantly improve the yield of your target protein,including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.

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    Service Advantages
  • Multiple expression systems: Sf9, Sf21, Hi5
  • One-stop service: from gene to protein expression purification
  • Fast delivery: minimum 4 weeks
  • Quality expression: provides protein at milligram to gram levels
  • Baculovirus-Insect Cell Protein Expression Service Details

    Service StepService   DescriptionTimelineDeliverable
    Gene synthesis and subclone  - Codon optimization and gene synthesis

      - Clone the   target gene into the vector plasmid

    1-2 weeks

      - Purified proteins

      - CoA 
    Construction of recombinant baculovirus vector  - Subcloning the target gene into a baculovirus transfer vector1-2 weeks

    Recombinant baculovirus expansion culture and

    protein expression and purification

      - Transfect insect cells, prepare viral stocks and expand cultures

      - Pilot expression and purification trial

      - QC analysis
    2-3 weeks

    Scale up expression and purification (optional)

      - Target protein

      - QC analysis
    3-5 weeks

    Baculovirus Protein Expression Service Process
  • Gene synthesis
  • Clone the target gene into the vector plasmid
  • Cloning the target gene into the baculovirus transfer vector by transposition
  • Transfection of insect cells and virus expansion
  • Small scale pilot expression and purification
  • Scale up expression and purification (optional)
  • Product delivery
  • Baculovirus Protein Expression Case
    Case #1: Express the target protein in Baculovirus-Insect expression system

    The target protein is expressed by Baculovirus-Insect expression system, the purified protein and quality  test report are successfully delivered. The relevant SDS-PAGE and SEC-HPLC analysis are shown in  Figure 1 and Figure 2.

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  • Phone: +86-571-88617879
  • Email: cro_service@clongene.com