Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. Compared to the E. coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. Clongene can provide high-quality experimental scheme and significantly improve the yield of your target protein，including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
| Gene synthesis and subclone | - Codon optimization and gene synthesis - Clone the target gene into the vector plasmid | 1-2 weeks | - Purified proteins - CoA |
| Construction of recombinant baculovirus vector | - Subcloning the target gene into a baculovirus transfer vector | 1-2 weeks | |
Recombinant baculovirus expansion culture and protein expression and purification | - Transfect insect cells, prepare viral stocks and expand cultures - Pilot expression and purification trial - QC analysis | 2-3 weeks | |
Scale up expression and purification (optional) | - Target protein - QC analysis | 3-5 weeks |
The target protein is expressed by Baculovirus-Insect expression system, the purified protein and quality test report are successfully delivered. The relevant SDS-PAGE and SEC-HPLC analysis are shown in Figure 1 and Figure 2.
