The mammalian cell expression has the advantage of guiding the correct folding of protein, providing complex glycosylation modification. Therefore, the expressed recombinant protein is closest to natural higher biological protein molecules in molecular structure, physicochemical properties and biological function, and has better activity than prokaryotic expression system and eukaryotic expression system such as yeast and insect cell. HEK293 cells and CHO cells were used as expression hosts in the mammalian transient expression system of Clongene. With years of experience in protein expression, Clongene can provide flexible services to meet the needs of different customers.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
Gene synthesis and subclone | - Codon optimization and gene synthesis - Subcloning into an expression vector - Plasmid amplification and preparation | 1-2 weeks | - Purified proteins - CoA |
| Protein expression and purification | - Transient transfection of HEK293/CHO cells - Protein purification - QC analysis | 2-3 weeks | |
| Large-scale expression and purification (Optional) | - Target protein - QC analysis | 3-5 weeks |
The mammalian transient expression system is used to express target protein. The SDS-PAGE of the purified protein is shown in Figure 1.
