The advantage of mammalian cell expression system is to guide the correct folding of protein, provide complex glycosylation modification, the expression of recombinant protein in molecular structure, physical and modification properties and biological function is closest to natural higher biological protein molecules, in terms of activity is far better than prokaryotic expression system and yeast, insect cell eukaryotic expression system. The mammalian transient expression system of Clongene uses HEK293 and CHO cells as expression hosts. With years of experience in protein expression, it can provide flexible services to meet the needs of different customers.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
Gene synthesis and subclone | - Codon optimization and gene synthesis - Subcloning into an expression vector - Plasmid amplification and preparation | 1-2 weeks | - Purified proteins - CoA |
| Protein expression and purification | - Transient transfection of HEK293/CHO cells - Protein purification - QC analysis | 2-3 weeks | |
| Large-scale expression and purification (Optional) | - Target protein - QC analysis | 3-5 weeks |
Use the mammalian cell transient expression system to express and purify the target protein. The SDS-PAGE is as follows:
