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    Your Position: Home > Services > Protein Expression Services > CHO-K1/CHO-GS Stable Cell Line Development
    CHO-K1/CHO-GS Stable Cell Line Development

    Stable cell line construction is the exogenous gene enters the recipient cell and integrates into the cell chromosome, so that the host cells can stably express the target gene in the long term. Stable cell lines are widely used in the fields of recombinant protein, antibody production, gene editing, functional studies and other fields. Clongene has been engaged in protein antibody expression research for many years, has a large amount of experience in the development of stable cell lines, and can provide diversified services to meet the needs of customers.

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    Service Advantages
  • Experience: more than 5 years of cell line development experience
  • High expression: more than 5 g/L monoclonal antibody
  • Stable and high yield: stable transmission for more than 30 generations
  • Fast screening: 3 months from DNA to cell line
  • CHO-K1 Stable Cell line Generation Details

    Service StepService   DescriptionTimelineDeliverable
    Gene synthesis and subclone

      - Codon optimization and gene synthesis

      - Subcloning into expression plasmids

      - Plasmid amplification and preparation
    2-3 weeks


    Transient expression evaluation   - Transient expression
      - Expression level detection by ELISA or WB
    2 weeks  - Evaluation report

    Stable pool generation

       - Stable transfection
      - Stable Pool selection 
    6-9 weeks

      - 2 vail Stable Pool cells

      - CoA 

    Single clone selection and screening

       - Subcloning

      - Single clone selection and screening
    6-9 weeks

      - A stable clone of a single cell origin

      - CoA
    Large-scale expression and purification

      - 100+ mg

      - QC analysis
    2-3 weeks

      - Purified proteins        

      - CoA

    CHO-K1 Stable Cell line Generation Workflow
  • Gene synthesis
  • Vector construction
  • Transient expression evaluation
  • Stable cell line generation selection and screening
  • Single clone selection and screening
  • Protein expression and purification
  • Product delivery
  • CHO-K1 Stable Cell line Service Case
    Case #1: Expression of the HP monoclonal antibody in the constructed CHO-K1 stable cell line

    Clungene constructed the CHO-K1 stable cell line which can express the HP monoclonal antibody. The  affinity test result after expression and purification is shown in Figure 1.

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    Contact Us
  • Phone: +86-571-88617879
  • Email: cro_service@clongene.com