Stable cell line construction is the exogenous gene enters the recipient cell and integrates into the cell chromosome, so that the host cells can stably express the target gene in the long term. Stable cell lines are widely used in the fields of recombinant protein, antibody production, gene editing, functional studies and other fields. Clongene has been engaged in protein antibody expression research for many years, has a large amount of experience in the development of stable cell lines, and can provide diversified services to meet the needs of customers.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
| Gene synthesis and subclone | - Codon optimization and gene synthesis - Subcloning into expression plasmids - Plasmid amplification and preparation | 2-3 weeks | - |
| Transient expression evaluation | - Transient expression - Expression level detection by ELISA or WB | 2 weeks | - Evaluation report |
Stable pool generation | - Stable transfection - Stable Pool selection | 6-9 weeks | - 2 vail Stable Pool cells - CoA |
| Single clone selection and screening | - Subcloning - Single clone selection and screening | 6-9 weeks | - A stable clone of a single cell origin - CoA |
| Large-scale expression and purification | - 100+ mg - QC analysis | 2-3 weeks | - Purified proteins - CoA |
Clungene constructed the CHO-K1 stable cell line which can express the HP monoclonal antibody. The affinity test result after expression and purification is shown in Figure 1.
