Mouse monoclonal antibody is used to fuse myeloma cells with activated B lymphocytes by cell technology to obtain hybridoma cells, which can secrete monoclonal antibody against a single antigen determination cluster. The antibodies have high specificity, stable passage, and can be large-scale repeated production, which is widely used in basic research, clinical diagnosis and new drug development.
With years of experience in monoclonal antibody research and development and a complete antibody production line, Clongene can provide you with one-stop customized services from antigen design, animal immunization, fusion screening to antibody production, and ultimately deliver high-quality monoclonal antibodies aligned with the client’s application requirements, and meeting clients’ different needs.
| Service Step | Service Description | Timeline | Deliverable |
Antigen preparation (optional) | - Customer provision/ Clongene preparation according to customer requirements | - Protein antigen: 3 weeks - Small molecule antigen: 4 weeks - Polypeptide antigen: 4 weeks | - Purity > 90% - Deliver antigen production in accordance with customer requirements - Test report |
Animal immunization | - 4-5 Balb/C mice - Detection of serum titer in mice | 3 weeks | - ELISA results of immune serum - Test report |
Cell fusion and screening | - Electrofusion - Clone screening | 4 weeks | - 1-3 positive clones (2 tubes of frozen cells per clone) - Test report |
Antibody preparation | - Preparation of ascites by intraperitoneal inoculation | 2 weeks | - 5-10 mg |
Antibody purification | - Octanoic acid-ammonium sulfate method /ProteinA/G method | 3 days | - 1-2 mg antibody - SDS - PAGE>95% - Test report |
The NP monoclonal antibody is prepared by hybridoma technique and purified by Protein A，the purified SDS-PAGE is shown in Figure 1. Its activity is analyzed by ELISA, and the result is shown in Figure 2. It can be seen from the results that NP antibody has high sensitivity and good activity.
