With the growing demand for research-type antibodies and the screening of a large number of antibody samples in a short time, Clongene has developed a high-throughput recombinant antibody production services to support the screening of antibody drugs. High-throughput recombinant antibody production services only take 18 working days from gene synthesis to antibody delivery. HEK293 cells or CHO cells are used as host cells to provide customers with fast and efficient recombinant antibody production services through standardized antibody expression platform.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
Gene synthesis and subclone (Customers can provide antibody sequences or plasmid as starting material) | - Codon optimization and gene synthesis - Subcloning into an expression vector - Plasmid amplification and preparation | 1-2 weeks | - Purified proteins - CoA |
| Transient expression and purification | - Transient transfection of cells - Antibody protein purification - QC analysis | 2-3 weeks |
According to the customer's requirements, we used the mammalian transient expression system to produce antibodies. The purified antibodies are verified by SDS-PAGE and SEC-HPLC.
