Clongene has developed a large-scale monoclonal antibody production (to kg) service, providing customers with recombinant antibodies in the amount of gram to kilogram. Clongene designs the protocol according to the scale specified by the customer, optimize the sequence design, culture protocol, transfection method and cell line construction protocol to meet customer's specific needs, and guarantee the delivery of the antibody of the expression amount and quality of customer's choice.
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
Gene synthesis and subclone (Customers can provide antibody sequences or plasmid as starting material) | - Codon optimization and gene synthesis - Subcloning into an expression vector - Plasmid amplification and preparation | 1-2 weeks | - Purified proteins - CoA |
| Small scale pilot expression and purification | - Transfection of mammalian cells - Small scale pilot expression and purification - QC analysis | 2-3 weeks | |
| Large-scale production (up to 250L) | - Large-scale plasmid preparation - Transient transfection and purification - QC analysis | 5-8 weeks |
Use Large-scale expression of recombinant antibody production service platform to express recombinant antibody.
The SDS-PAGE is as follows:
